Scientific Program

Program

Keynote Talk

Jan Huisken. University of Göttingen. Germany

FLAMINGO: Multiscale Lightsheet microscopy inside and outside the optics lab.

Session I: Mesoscopy and Organism imaging

Laura Batti. Wyss Center. Switzerland

“Plunge” into the brain using Lightsheet microscopy.

Selected speakers

Julien Colombelli. IRB Barcelona. Spain

Hyperpolarized scattered Lightsheet microscopy for label free cleared tissue imaging.

Emilio J. Gualda. Universitat Politècnica de Catalunya. Spain

Development of microscopic techniques (LSFM, OPT and tissue clearing) for the visualization of plant–root-knot nematode interaction.

Antonio Martínez Sielva. Universidad de Castilla-La Mancha. Spain

Imaging calcium in the beating heart of zebrafish larvae: influence of mechano-electrical feedback.

Session II: Functional imaging

Clara Sánchez Rodríguez. Swiss Federal Institute of Technology Zürich. Switzerland

Diving into plant biology through in vivo Spinning Disc Confocal microscopy.

Yolanda R. Carrasco. Centro Nacional de Biotecnología. Spain

Applying functional microscopy to dissect B lymphocyte dynamics and the undelying molecular mechanisms.

Selected speakers

José Javier Ruiz. Institut de Ciències Fotòniques. Spain

Label-free characterization of breast cancer cells resistant to neoadjuvant treatments by Raman micro-spectroscopy imaging.

Margarita Ferriz. Centro Nacional de Biotecnología. Spain

Imaging of the peritoneal cavity in mice during infection and metastatic tumor growth.

Session III: Super-resolution microscopy

Peter Dedecker. KU Leuven. Belgium

More informative imaging with ‘smart’ probes and PSF engineering.

Jan Tonnesen. Achucarro Basque Center for Neuroscience. Spain

Imaging the neuropil in live mouse brain slices by STED microscopy.

Selected speakers

Xavier Casas Moreno. KTH Royal Institute of Technology. Sweden

Software and hardware integration for high-throughput nanoscopy.

David Valdivieso González. Universidad Complutense de Madrid. Spain

Expansion microscopy of biofilms from nosocomial pathogens.

Javier Díez Guerra. Centro de Biología Molecular Severo Ochoa. Spain

Expansion microscopy: application to the localization of proteins in the synaptic environment.

Session IV: Functional imaging at subcellular resolution

Ángel Orte. Universidad de Granada. Spain

Advanced time-resolved and multiparametric microscopy for FRET/FLIM and beyond.

Carlo Manzo. Universitat de Vic. Spain

Data-driven approaches for the analysis of molecular motion.

Simão Instituto Gulbenkian de Ciência. Portugal

Volumetric light-sheet imaging.

Selected speakers:

Antonio Lorca Cámara. Institut de la Vision. France

A flexible Two-Photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals.

Arturo G. Vesga. Centro Nacional de Biotecnología. Spain

FCS and single molecule tracking to quantify interactions between protein and lipid membranes.

María Leonor Ribeiro. International Iberian Nanotechnology Laboratory. Portugal

Metabolic imaging of 2D and 3D live cell models using a beam-scanning SYNC-RGB-FLIM microscope.

Session V: Image and data analysis

Gloria Bueno. Universidad de Castilla-La Mancha. Spain

Low-cost microscopy system for river and ocean diatom detection.

Arrate Muñoz-Barrutia. Universidad Carlos III de Madrid. Spain

Microscopy image analysis to support discoveries in life sciences.

Selected speakers

Santiago Fernández González. Institute for Research in Biomedicine. Switzerland

Immunemap, an open data platform to enable large-scale, image-driven immunological research.

Jorge Valero. Instituto de Neurociencias de Castilla y León. Spain

VOLUMESTJ: a new tool for volumetric estimation in tissue sections.

Gemma Martín. Universitat de Barcelona. Spain

DISJ: new tool to analyze organelles and cell dispersion, distribution and distances in light microscopy imaging.

Pau Carrillo-Barberà. Universitat de València. Spain

High content screening to quantify morphometric features on 3D cultures.

Session VI: Correlative Microscopy

José Javier Conesa. Centro Nacional de Biotecnología. Spain

Cryo-correlative light and electron microscopy.

Mónica Fernández Monreal. Bordeaux Imaging Center. France

Correlative light and electron Microscopy (CLEM).

Selected speakers

Blanca Soler. Centro Nacional de Biotecnología. Spain

Growth hormone remodels the 3D-ultrastructure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming.

Felipe Viela. IMDEA Nanociencia. Spain

Real-time imaging of the mechanobactericidal action of nanomaterials.

Community Workshops

Michael Weber & Jan Huisken. University of Göttingen. Germany

Hands-on Lightsheet microscopy with Flamingo, the modular and shareable research microscope.

Marta Monteiro & Nuno Moreno. Instituto Gulbenkian de Ciência. Portugal.

Manel Bosch. Centres Científics i Tecnològics. Universitat de Barcelona. Spain

Inteligent core facility management.

Luisa Cortes & Gaby Martins. Center for Neuroscience and Cell Biology & Instituto Gulbenkian de Ciência. Portugal

Sample clearing methodologies.

Gianluca D’Agostino & Ana Oña. Centro Nacional de Biotecnología. Spain

Keys to STED and STORM sample preparation.

José Javier Conesa

Correlative microscopy.

Ana Cayuela & Carlos Óscar Sorzano. Centro Nacional de Biotecnología. Spain

Recurrent image processing tasks in cell and molecular biology using ImageJ.

Posters Flash Talks

Pablo Delgado-Rodríguez. Universidad Carlos III de Madrid. Spain

DL-based classification of abnormal mitoses on radiated cells.

Jorge Castro-Sangrador. Universidad de Salamanca. Spain

PDK1 kinase functions in Schizosaccaromyces pombe mitosis and cytokinesis.

Laura Nicolás-Sáenz. Universidad Carlos III de Madrid. Spain

ABANICCO: A new method for unsupervised color segmentation.

José João Vitória. Universidade de Aveiro. Portugal

Optimising the automated analysis of live-imaged mitochondria with the MATLAB tool Mitometer.

Tomás Pais de Azevedo. Algarve Biomedical Center Research Institute. Portugal

Using time-lapse imaging to unravel the dynamics of early chicken embryo elongation.

David Delgado Gestoso. Centro Nacional de Biotecnología. Spain

Cryo-correlative platform in the CRYOEM-CNB facility.

Ana Cayuela. Centro Nacional de Biotecnología. Spain

Flexible single particle tracking and analysis in optical microscopy.

Platinum Sponsor Talk

Kirstin Elgass. Application Development Engineer

ZEISS Elyra 7 with Lattice SIM2. Your Live Imaging System with Unprecedented Resolution.

Tech Bites

Platinum sponsor ZEISS

ZEISS cutting-edge Microscopy.

Gold sponsor Izasa Scientific

New approaches in confocal and label-free imaging.

Gold sponsor Paralab Bio

Paralab BIO – Cutting Edge Technologies for Biosciences

Industry Workshops

• Platinum sponsor ZEISS

Kirstin Elgass. Application Development Engineer

LLS7 and LS 7: New era of volumetric live cell imaging.

Soren Prag. Product & Application Sales Specialist

Celldiscoverer 7: Perform Automated Gentle and Fast Confocal 3D Imaging.

Delisa García. Head of Direct Sales Imaging for EMEA and ROW

Arivis: AI approaches to Image Analysis and Neuron Analysis for Big Data.

 

• Gold sponsor Izasa Scientific

Alexandra Schroeder & Zuzana Nováková. Application Specialists Telight

LiveCodim: Conical diffraction microscopy for super-resolution live cell imaging & Quantitative Phase Imaging: pictures where every picogram counts.

Herman Fennema. Application Specialist Nikon

Expand to lifetime imaging: A seamless integration of AX point scanner confocal and FLIM.

Benoit Binctin. Sales Engineer Andor

BC43 – The benchtop confocal by Andor – compact, fast, easy and superb image quality at affordable cost.

 

• Gold sponsor Paralab Bio

Adolfo Molejón, Product Manager Paralab Bio

Real-time IntraVital Microscopy (IVM): In Vivo Cellular-level Imaging of Internal Organs in a Live Animal.

Martin Humphry. CEO Phasefocus

From 96-wells to single cells: Introduction to high content live cell assays on Livecyte.

Beniamino Barbieri. President ISS

Microscopy Tools for Quantitative Cell Biology with Single-molecule Sensitivity

 

• Silver sponsor Evident Olympus

Stefan Marawske. Sales & Application Specialist

IXplore SpinSR: The big picture of small things – Intelligent sample detection

 

• Silver sponsor Leica microsystems

Jens Peter Gabriel. Advanced Workflow Specialist EMEA, Confocal Microscopy

Explore new dimensions with STELLARIS: How your imaging applications can benefit from fluorescence lifetime-based information.

 

• Silver sponsor Miltenyi Biotec

Luis Miguel Muñiz Menéndez. Imaging Sales Specialist

Full context microscopy: the MACSimaTM platform.

 

• Silver sponsor Thermo Fisher Scientific

Sarawuth Wantha. Product Applications Specialist

Advanced imaging data analysis and visualization for optical microscopy.

Calendar

DAY 1 – Wednesday, 23rd November

8:00 – 08:50Registration + Poster setup
8:50 – 09:00Opening
9:00 – 10:00Keynote Lecture
FLAMINGO: Multiscale Light Sheet microscopy inside and outside the optics lab
Jan Huisken. University of Göttingen. Germany
10:00 – 10:30Session I: Mesoscopy and Organism imaging
Chairman: Gaby G. Martins. Instituto Gulbenkian de Ciência (IGC). Portugal

Invited speaker

“Plunge” into the brain using lightsheet microscopy
Laura Batti. Wyss Center. Switzerland
10:30 – 11:00Coffee break + Posters
11:00 – 11:45Session I: Mesoscopy and Organism imaging
11:00 Hyperpolarized scattered Lightsheet microscopy for label free cleared tissue imaging.
Julien Colombelli. Institute for Research in Bioimedicine Barcelona. Spain
11:15 Development of microscopic techniques (LSFM, OPT and tissue clearing) for the visualization of plant–root-knot nematode interaction.
Emilio J. Gualda. Universitat Politècnica de Catalunya. Spain
11: 30 Imaging calcium in the beating heart of zebrafish larvae: influence of mechano-electrical feedback.
Antonio Martínez Sielva. Centro Regional de Investigaciones Biomédicas (CRIB)/Universidad de Castilla-La Mancha. Spain
11:45 – 12:15Posters Flash Talks (From P1 to P7)
Chairman: Julien Colombelli. Institute for Research in Biomedicine
(IRB Barcelona). Spain

PDK1 kinase functions in Schizosaccaromyces pombe mitosis and cytokinesis.
Jorge L. Castro-Sangrador. CSIC-Universidad de Salamanca. Spain
Using time-lapse imaging to unravel the dynamics of early chicken embryo elongation.
Tomás Pais de Azevedo. Algarve Biomedical Center Research Institute. Portugal
Cryo-correlative platform in the CRYOEM-CNB facility.
David Delgado Gestoso. Centro Nacional de Biotecnología (CNB). Spain
DL-based classification of abnormal mitoses on radiated cells
Pablo Delgado-Rodríguez. Universidad Carlos III de Madrid. Spain
ABANICCO: A new method for unsupervised color segmentation.
Laura Nicolás-Sáenz. Universidad Carlos III de Madrid. Spain
Optimising the automated analysis of live-imaged mitochondria with the MATLAB tool Mitometer.
José João Vitória. Universidade de Aveiro. Portugal
Flexible single particle tracking and analysis in optical microscopy.
Ana Cayuela
. Centro Nacional de Biotecnología (CNB). Spain

12:15 – 13:05Industry Workshops (parallel x5)
Platinum sponsor ZEISS
LLS7 and LS 7: New era of volumetric live cell imaging.
Kirstin Elgass. Application Development Engineer
Gold sponsor Izasa Scientific
LiveCodim: Conical diffraction microscopy for super-resolution live cell imaging & Quantitative Phase Imaging: pictures where every picogram counts.
Alexandra Schroeder & Zuzana Nováková, Application Specialists Telight
Gold sponsor Paralab Bio
From 96-wells to single cells: Introduction to high content live cell assays on Livecyte.
Martin Humphry. CEO Phasefocus
Silver sponsor Evident Olympus
IXplore SpinSR: The big picture of small things – Intelligent sample detection
Stefan Marawske. Sales & Application Specialist

Silver sponsor Leica Microsystems
Explore new dimensions with STELLARIS: How your imaging applications can benefit from fluorescence lifetime-based information.
Jens Peter Gabriel, Advanced Workflow Specialist EMEA, Confocal Microscopy
13:05 – 14:30Lunch
14:30 – 16:00Session II: Functional imaging
Chairwoman: María Calvo. Centros Científicos y Tecnológicos de la Universidad de Barcelona (CCiTUB). Spain

Invited speakers
14:30 Diving into plant biology through in vivo spinning disc confocal microscopy
Clara Sánchez Rodríguez. Swiss Federal Institute of Technology Zürich. Switzerland
15:00 Applying functional microscopy to dissect B lymphocyte dynamics and the undelying molecular mechanisms
Yolanda R. Carrasco. Centro Nacional de Biotecnología (CNB). Spain
Selected speakers
15:30 Label-free characterization of breast cancer cells resistant to neoadjuvant treatments by Raman micro-spectroscopy imaging.
José Javier Ruiz. Institut de Ciències Fotòniques (ICFO). Spain
15:45 Imaging of the peritoneal cavity in mice during infection and metastatic tumor growth.
Margarita Ferriz. Centro Nacional de Biotecnología (CNB). Spain
16:00 – 16:30Coffee break + Posters
16:30 – 18:30Community Workshops (parallel x2)
16:30 *Hands-on Lightsheet microscopy with Flamingo, the modular and shareable research microscope
Michael Weber & Jan Huisken. University of Göttingen. Germany
*Inteligent Core Facility Management
Marta Monteiro & Nuno Moreno. Instituto Gulbenkian de Ciência. Portugal
17:30 *Sample clearing methodologies
Luisa Cortes. Center for Neuroscience and Cell Biology. Portugal &
Gaby Martin. Instituto Gulbenkian de Ciência. Portugal
Manel Bosch. Centres Científics i Tecnològics. Universitat de Barcelona. Spain
*Keys to STED and STORM sample preparation
Gianluca D’Agostino and Ana Oña. Centro Nacional de Biotecnología (CNB). Spain
18:30 – 19:30Welcome reception + Posters

DAY 2 – Thursday, 24th November

8:30 – 09:00Registration
9:00 – 10:45Session III: Super-resolution microscopy
Chairman: José Requejo-Isidro. Centro Nacional de Biotecnología (CNB). Spain
Invited speakers
9:00 Unconventional fluorophores for super-resolution microscopy
Cristina Flors. Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia). Spain
9:30 Imaging the neuropil in live mouse brain slices by STED microscopy
Jan Tonnesen. Achucarro Basque Center for Neuroscience. Spain
Selected speakers
10:00 Software and hardware integration for high-throughput nanoscopy.
Xavier Casas Moreno. KTH Royal Institute of Technology. Sweden
10:15 Expansion microscopy of biofilms from nosocomial pathogens.
David Valdivieso González. Universidad Complutense de Madrid. Spain
10:30 Expansion microscopy: application to the localization of proteins in the synaptic environment.
F. Javier Díez Guerra. Centro de Biología Molecular Severo Ochoa (CBMSO). Spain
10:45 – 11:00Tech Bites
Chairman: Julien Colombelli. Institute for Research in Biomedicine
(IRB Barcelona). Spain
10:45 ZEISS cutting-edge Microscopy.
Platinum sponsor ZEISS
10:50 Paralab BIO – Cutting Edge Technologies for Biosciences.
Gold sponsor Paralab Bio
10:55 New approaches in confocal and label-free imaging.
Gold sponsor Izasa Scientific
11:00 – 11:30Coffee break + Posters
11:30 – 12:20Industry Workshops (parallel x5)
Platinum sponsor ZEISS
Arivis: AI approaches to Image Analysis and Neuron Analysis for Big Data.
Delisa García. Head of Direct Sales Imaging for EMEA and ROW
Gold sponsor Izasa Scientific
Expand to lifetime imaging: A seamless integration of AX point scanner confocal and FLIM.
Herman Fennema. Application Specialist Nikon
Gold sponsor Paralab Bio
Real-time IntraVital Microscopy (IVM): In Vivo Cellular-level Imaging of Internal Organs in a Live Animal.
Adolfo Molejón. Product Manager Paralab Bio
Silver sponsor Evident Olympus
IXplore SpinSR: The big picture of small things – Intelligent sample detection
Stefan Marawske. Sales & Application Specialist
Silver sponsor Miltenyi Biotec
Full context microscopy: the MACSimaTM platform.
Luis Miguel Muñiz Menéndez. Imaging Sales Specialist
12:20 – 13:20Community Workshops (parallel x2)
*Recurrent image processing tasks in cell and molecular biology using Image
Ana Cayuela & Carlos Óscar Sorzano. Centro Nacional de Biotecnología (CNB). Spain
*Correlative Microscopy
José Javier Conesa. Centro Nacional de Biotecnología (CNB). Spain
13:20 – 14:30Lunch
14:30 – 15:20Industry Workshops (parallel x4)
Platinum sponsor ZEISS
Celldiscoverer 7: Perform Automated Gentle and Fast Confocal 3D Imaging.
Soren Prag. Product & Application Sales Specialist
Gold sponsor Izasa Scientific
BC43 – The benchtop confocal by Andor – compact, fast, easy and superb image quality at affordable cost.
Benoit Binctin. Sales Engineer Andor
Gold sponsor Paralab Bio
Microscopy Tools for Quantitative Cell Biology with Single-molecule Sensitivity
Beniamino Barbieri. President ISS
Silver sponsor Thermo Fisher Scientific
Advanced imaging data analysis and visualization for optical microscopy.
Sarawuth Wantha. Product Applications Specialist
15:20 – 16:20Session IV: Functional imaging at subcellular resolution

Chairwoman: Cristina Flors. Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia). Spain
Invited speakers
15:20 Advanced time-resolved and super-resolution microscopy
Ángel Orte. Universidad de Granada. Spain
15:50 Data-driven approaches for the analysis of molecular motion
Carlo Manzo. Universitat de Vic. Spain
16:20 – 16:50Coffee break + Posters
16:50 – 17:10Platinum Sponsor Talk
ZEISS Elyra 7 with Lattice SIM2. Your Live Imaging System with Unprecedented Resolution.
Kirstin Elgass. Application Development Engineer
17:10 – 18:25Session IV: Functional imaging at subcellular resolution
Chairwoman: Cristina Flors. Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia). Spain
Invited speaker
17:10 Volumetric light-sheet imaging.
Simão Coelho. Instituto Gulbenkian de Ciência. Portugal
Selected speakers
17:40 A flexible Two-Photon endoscope for fast activity imaging and cell precise optogenetic photo-stimulation of neurons in freely moving animals.
Antonio Lorca Cámara. Sorbonne Université/INSERM/CNRS/Institut de la Vision. France
17:55 FCS and single molecule tracking to quantify interactions between protein and lipid membranes.
Arturo G. Vesga. Centro Nacional de Biotecnología (CNB). Spain
18:10 Metabolic imaging of 2D and 3D live cell models using a beam scanning SYNC-RGB-FLIM microscope.
María Leonor Ribeiro. International Iberian Nanotechnology Laboratory (INL). Portugal
18:30 – 19:30REMOA PPBI Meeting
20:30Dinner

DAY 3 – Friday, 25th November

9:00 – 11:00Session V: Image analysis and data
Chairman: Carlos Óscar Sorzano. Centro Nacional de Biotecnología (CNB). Spain

Invited speakers
9:00 Low-cost microscopy system for river and ocean diatom detection
Gloria Bueno. Universidad de Castilla-La Mancha. Spain
9:30 Microscopy image analysis to support discoveries in life sciences
Arrate Muñoz-Barrutia. Universidad Carlos III de Madrid. Spain
Selected speakers
10:00 Immunemap, an open data platform to enable large-scale, image driven immunological research.
Santiago Fernández González. Institute for Research in Biomedicine. Switzerland
10:15 VOLUMESTJ: a new tool for volumetric estimation in tissue sections.
Jorge Valero. Instituto de Neurociencias de Castilla y León (INCYL)/Universidad de Salamanca. Spain
10:30 DISJ: new tool to analyze organelles and cell dispersion, distribution and distances in light microscopy imaging.
Gemma Martín. Centros Científicos y Tecnológicos de la Universidad de Barcelona. Spain
10:45 High content screening to quantify morphometric features on 3D cultures.
Pau Carrillo-Barberà. Instituto de Biotecnología y Biomedicina (BioTecMed)/Universitat de València. Spain

11:00 – 11:30Coffee break
11:30 – 13:00Session VI: Correlative Microscopy
Chairman: Gianluca D’Agostino. Centro Nacional de Biotecnología (CNB). Spain

Invited speakers
11:30 Cryo-correlative light and electron microscopy
José Javier Conesa. Centro Nacional de Biotecnología (CNB). Spain
12:00 Correlative light and electron Microscopy (CLEM)
Mónica Fernández Monreal. Bordeaux Imaging Center. France
Selected speakers
12:30 Growth hormone remodels the 3D-ultrastructure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming
Blanca Soler Palacios. Centro Nacional de Biotecnología (CNB). Spain
12:45 Real-time imaging of the mechanobactericidal action of nanomaterials
Felipe Viela. Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia). Spain
13:00 – 13:15Closing remarks & Awards
13:15 – 13:30Meeting end & Lunch box